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bosborne
Joined: 13 Jun 2008
Posts: 2
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 Posted: 13 Jun 2008 6:53 pm
Post subject: Residual acrylamide in wells
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Anyone have the issue of residual acrylamide left in wells after pulling the comb from a gel? It's as if the tolerance of the comb thickness is not that well controlled. Any tips as to how to eliminate this (aside from buying the precast gels)?
Thanx! 
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Steffen-ac
Joined: 16 Jun 2008
Posts: 1
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Posted: 16 Jun 2008 11:21 am
Post subject: Re: Residual acrylamide in wells
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| bosborne wrote: | Anyone have the issue of residual acrylamide left in wells after pulling the comb from a gel? It's as if the tolerance of the comb thickness is not that well controlled. Any tips as to how to eliminate this (aside from buying the precast gels)?
Thanx! |
Hi,
make sure that you use dry combs. I often got that problem, if i use wet combs.
In addition we polymerize our gels at 37°C.
Give it a try.
Greetings from Germany!
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AntPan
Joined: 14 May 2008
Posts: 4
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Posted: 23 Jun 2008 3:26 pm
Post subject:
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You can try rinsing your wells with water after removing the comb...this is what we do in our lab and it seems to work!
Good luck
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sazzal99
Joined: 10 Apr 2008
Posts: 11
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Posted: 24 Jul 2008 11:20 am
Post subject:
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we too rinse wells with buffer (and use dry combs) and do not experience this problem
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dixy
Joined: 04 May 2010
Posts: 5
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Posted: 07 May 2010 10:32 pm
Post subject:
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1. Use a dry comb and also dry and clean plates;
2. Take a syringe with a needle and fill it with buffer-,
3. Insert the needle tip in side a well (do not puncture it) and wash by pressing the syringe plunger multiple times. Press well so u get a "jet" out of the needle.U will see unpolymerized AA swimming out the well into the buffer reservoir.
4. Repeat multiple times washing untill u see no AA swimming out;
Enjoy your electrophoresis 
Many regards
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AKCanyonKing
Joined: 11 Jun 2010
Posts: 4
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Posted: 11 Jun 2010 6:17 am
Post subject: Suggestions
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I too had this problem...thanks for the input
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Drieshyaresty
Joined: 17 Jul 2010
Posts: 2
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Posted: 29 Jul 2010 5:33 am
Post subject: Residual acrylamide in wells
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Very useful piece
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