mytetracell.com

Instructional Video

electroboy — 20 Nov 2008 4:40 pm

Author: electroboy

Posted: 20 Nov 2008 4:40 pm

Possible reason you are able to see video:

- You do not have Flash installed in your desktop/laptop

- You're version of Flash is out dated.

Link to Flash:
http://get.adobe.com/flashplayer/

Here I am !

electroboy — 18 Nov 2008 5:57 pm

Author: electroboy

Posted: 18 Nov 2008 5:57 pm

Great so what are you up to?

-e

Gel squeezing

BRJeff — 14 Nov 2008 10:06 pm

Author: BRJeff

Posted: 14 Nov 2008 10:06 pm

Better yet, pour some gels (or get some undergrad to do it for you) and try those out and see if you still have the same problem.

Western blot - Stripping and Reprobing

BRJeff — 14 Nov 2008 10:04 pm

Author: BRJeff

Posted: 14 Nov 2008 10:04 pm

eh, done that before and always got crappy blots. If your sample is precious, you could do a fluorescent western blot (given you have an imager).

Wavy gel pattern!

sazzal99 — 14 Nov 2008 4:17 pm

Author: sazzal99

Posted: 14 Nov 2008 4:17 pm

Top list!!

It can be lots of different things - I would make up fresh gels with fresh reagents as your first point of call!

stacking and resolving gels not 'sticking'

sazzal99 — 14 Nov 2008 4:15 pm

Author: sazzal99

Posted: 14 Nov 2008 4:15 pm

We always cast our gels all in the same day too, and never have any issue doing this way

Not sure why this may be happening, but your resolving gel may be drying out a little, leaving an uneven surface for your stacking gel?

Gel Staining

sazzal99 — 14 Nov 2008 4:13 pm

Author: sazzal99

Posted: 14 Nov 2008 4:13 pm

me too - I always add etBr to the gel before casting

x

Semi-Dry Transfer large proteins

Dt Spot — 12 Nov 2008 11:49 am

Author: Dt Spot

Posted: 12 Nov 2008 11:49 am

I've never worked with big proteins, but i've a good experience with smalls, and if you wanna use a semi-dry system i'd recommend to try a discontinuous buffer

http://www.biocompare.com/Articles/TechnicalArticle/1180/Increased-Transfer-Efficiency-Using-A-Discontinuous-Buffer-System-With-The-Trans-Blot-sup-SD-Cell-from-Bio-Rad.html

Good luck

Best sci-Fi Book

electroboy — 10 Nov 2008 11:20 pm

Author: electroboy

Posted: 10 Nov 2008 11:20 pm

Well that is a whole other topic. Do you actually have a book suggestion, if so lets here it.

-r

New Categories - Let us know.

mytetracell_admin — 31 Oct 2008 4:28 pm

Author: mytetracell_admin

Posted: 31 Oct 2008 4:28 pm

Thanks for all your suggestions, I have created new topics based on your suggestions. So lets start populating them!!!

-mtc

Quiz

mytetracell_admin — 31 Oct 2008 4:23 pm

Author: mytetracell_admin

Posted: 31 Oct 2008 4:23 pm

Yes agreed busy coming up with content that must of slipped. We are thinking of having a community contest to come up with next months questions of course will receive some freebies from the store!

-mtc

Competition winners

iduffy — 30 Oct 2008 9:12 am

Author: iduffy

Posted: 30 Oct 2008 9:12 am

I hope that didnt sound desperate or something??!!

Just by chance my 7 yr old had hijacked my iPod and made it a refuge of Jonas Brothers, Camp Rock, iCarly, etc. I was looking to purchase a new one for myself but since BioRad were good enough to offer me one ................

One cassette works, the other doesn't

kathleend — 28 Oct 2008 4:09 pm

Author: kathleend

Posted: 28 Oct 2008 4:09 pm

We had the same issue in our lab and it constantly leaked; Bio-Rad didn't have any solutions for the issue - good luck!

NIH grants

brichhardson — 21 Oct 2008 4:13 am

Author: brichhardson

Posted: 21 Oct 2008 4:13 am

So last week, Nature announced that the NIH will no longer be allowing proposal submissions for a third time. Researchers can still do an initial submission, and then one, ,but not a second.

What do people think?

It sort of worries me, my lab has an R01 that we've already had to submit a second time, and we haven't heard back. It is the biggest grant of the lab too.

PCR acronyms

brichhardson — 21 Oct 2008 4:09 am

Author: brichhardson

Posted: 21 Oct 2008 4:09 am

As great as PCR is, it can be a bitch.

So here, lets collect any and all alternate acronyms for PCR.

1) Pippette, cry, repeat.